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Proteintech muc2
Muc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+muc2/MUC2+Antibody/pm41897943-88-10-16
Average 96 stars, based on 261 article reviews
muc2 - by Bioz Stars, 2026-09
96/100 stars

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Blocking Assay:

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway.
Article Snippet: .. After cooling to room temperature (RT), sections were treated with 3% H2O2 for 20 min to block endogenous peroxidase, followed by 10% goat serum blocking at 37 °C for 1 h. Subsequently, they were incubated overnight at 4 °C with the primary antibodies: anti-CDX2 (Proteintech, #82,659–1- RR, Wuhan, China, 1:2000) or anti-MUC2 (Proteintech, #27675–1-AP, Wuhan, China, 1:2000). .. On the next day, sections underwent a 1-h incubation at RT with HRPconjugated Goat anti-Rabbit IgG (H + L) (Servicebio, #GB23303, Wuhan, China, 1:200).

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway.
Article Snippet: .. After rinsing, the cells were fixed with 4% PFA at RT for 30 min. For intracellular targets, cells were permeabilized with 1–5% Triton (Biosharp, China) for 15 min. 10% goat serum was applied to block non-specific binding at 37 °C for 30 min. Primary antibodies, including anti-Villin1 (Proteintech, #16,488–1-AP, Wuhan, China, 1:250), anti-KLF4 (Proteintech, #11,880–1-AP, Wuhan, China, 1:200), antiCDX2 (Immunoway, #YM3057, Jiangsu, China, 1:200), and anti-MUC2 (Proteintech, #27,675–1-AP, Wuhan, China, 1:400), were applied, and plates were sealed with parafilm and incubated overnight at 4 °C. .. The next day, cells were incubated in the dark for 1 h with Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, #A-11008, USA, 4 μg/mL) or Alexa Fluor 594-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, #A-11005, USA, 5 μg/mL) cross-adsorbed secondary antibody.

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway
Article Snippet: .. After rinsing, the cells were fixed with 4% PFA at RT for 30 min. For intracellular targets, cells were permeabilized with 1–5% Triton (Biosharp, China) for 15 min. 10% goat serum was applied to block non-specific binding at 37 °C for 30 min. Primary antibodies, including anti-Villin1 (Proteintech, #16,488–1-AP, Wuhan, China, 1:250), anti-KLF4 (Proteintech, #11,880–1-AP, Wuhan, China, 1:200), anti-CDX2 (Immunoway, #YM3057, Jiangsu, China, 1:200), and anti-MUC2 (Proteintech, #27,675–1-AP, Wuhan, China, 1:400), were applied, and plates were sealed with parafilm and incubated overnight at 4 °C. .. The next day, cells were incubated in the dark for 1 h with Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, #A-11008, USA, 4 μg/mL) or Alexa Fluor 594-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, #A-11005, USA, 5 μg/mL) cross-adsorbed secondary antibody.

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway
Article Snippet: .. After cooling to room temperature (RT), sections were treated with 3% H 2 O 2 for 20 min to block endogenous peroxidase, followed by 10% goat serum blocking at 37 °C for 1 h. Subsequently, they were incubated overnight at 4 °C with the primary antibodies: anti-CDX2 (Proteintech, #82,659–1-RR, Wuhan, China, 1:2000) or anti-MUC2 (Proteintech, #27675–1-AP, Wuhan, China, 1:2000). .. On the next day, sections underwent a 1-h incubation at RT with HRP-conjugated Goat anti-Rabbit IgG (H + L) (Servicebio, #GB23303, Wuhan, China, 1:200).

Incubation:

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway.
Article Snippet: .. After cooling to room temperature (RT), sections were treated with 3% H2O2 for 20 min to block endogenous peroxidase, followed by 10% goat serum blocking at 37 °C for 1 h. Subsequently, they were incubated overnight at 4 °C with the primary antibodies: anti-CDX2 (Proteintech, #82,659–1- RR, Wuhan, China, 1:2000) or anti-MUC2 (Proteintech, #27675–1-AP, Wuhan, China, 1:2000). .. On the next day, sections underwent a 1-h incubation at RT with HRPconjugated Goat anti-Rabbit IgG (H + L) (Servicebio, #GB23303, Wuhan, China, 1:200).

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway.
Article Snippet: .. After rinsing, the cells were fixed with 4% PFA at RT for 30 min. For intracellular targets, cells were permeabilized with 1–5% Triton (Biosharp, China) for 15 min. 10% goat serum was applied to block non-specific binding at 37 °C for 30 min. Primary antibodies, including anti-Villin1 (Proteintech, #16,488–1-AP, Wuhan, China, 1:250), anti-KLF4 (Proteintech, #11,880–1-AP, Wuhan, China, 1:200), antiCDX2 (Immunoway, #YM3057, Jiangsu, China, 1:200), and anti-MUC2 (Proteintech, #27,675–1-AP, Wuhan, China, 1:400), were applied, and plates were sealed with parafilm and incubated overnight at 4 °C. .. The next day, cells were incubated in the dark for 1 h with Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, #A-11008, USA, 4 μg/mL) or Alexa Fluor 594-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, #A-11005, USA, 5 μg/mL) cross-adsorbed secondary antibody.

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway
Article Snippet: .. After rinsing, the cells were fixed with 4% PFA at RT for 30 min. For intracellular targets, cells were permeabilized with 1–5% Triton (Biosharp, China) for 15 min. 10% goat serum was applied to block non-specific binding at 37 °C for 30 min. Primary antibodies, including anti-Villin1 (Proteintech, #16,488–1-AP, Wuhan, China, 1:250), anti-KLF4 (Proteintech, #11,880–1-AP, Wuhan, China, 1:200), anti-CDX2 (Immunoway, #YM3057, Jiangsu, China, 1:200), and anti-MUC2 (Proteintech, #27,675–1-AP, Wuhan, China, 1:400), were applied, and plates were sealed with parafilm and incubated overnight at 4 °C. .. The next day, cells were incubated in the dark for 1 h with Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, #A-11008, USA, 4 μg/mL) or Alexa Fluor 594-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, #A-11005, USA, 5 μg/mL) cross-adsorbed secondary antibody.

Article Title: Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A.
Article Snippet: .. The sections were then blocked with 3% bovine serum albumin (BSA) and incubated with the following primary antibodies: anti-Muc2 (Proteintech, 27675-1-AP, 1:500), anti-Ki67 (Servicebio, GB111402, 1:500), anti-Occludin (Servicebio, GB111401, 1:500). ..

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway
Article Snippet: .. After cooling to room temperature (RT), sections were treated with 3% H 2 O 2 for 20 min to block endogenous peroxidase, followed by 10% goat serum blocking at 37 °C for 1 h. Subsequently, they were incubated overnight at 4 °C with the primary antibodies: anti-CDX2 (Proteintech, #82,659–1-RR, Wuhan, China, 1:2000) or anti-MUC2 (Proteintech, #27675–1-AP, Wuhan, China, 1:2000). .. On the next day, sections underwent a 1-h incubation at RT with HRP-conjugated Goat anti-Rabbit IgG (H + L) (Servicebio, #GB23303, Wuhan, China, 1:200).

Binding Assay:

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway.
Article Snippet: .. After rinsing, the cells were fixed with 4% PFA at RT for 30 min. For intracellular targets, cells were permeabilized with 1–5% Triton (Biosharp, China) for 15 min. 10% goat serum was applied to block non-specific binding at 37 °C for 30 min. Primary antibodies, including anti-Villin1 (Proteintech, #16,488–1-AP, Wuhan, China, 1:250), anti-KLF4 (Proteintech, #11,880–1-AP, Wuhan, China, 1:200), antiCDX2 (Immunoway, #YM3057, Jiangsu, China, 1:200), and anti-MUC2 (Proteintech, #27,675–1-AP, Wuhan, China, 1:400), were applied, and plates were sealed with parafilm and incubated overnight at 4 °C. .. The next day, cells were incubated in the dark for 1 h with Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, #A-11008, USA, 4 μg/mL) or Alexa Fluor 594-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, #A-11005, USA, 5 μg/mL) cross-adsorbed secondary antibody.

Article Title: Weiyan Tongluo Granules attenuate gastric intestinal metaplasia through PPARγ/NF-κB/CDX2 signaling pathway
Article Snippet: .. After rinsing, the cells were fixed with 4% PFA at RT for 30 min. For intracellular targets, cells were permeabilized with 1–5% Triton (Biosharp, China) for 15 min. 10% goat serum was applied to block non-specific binding at 37 °C for 30 min. Primary antibodies, including anti-Villin1 (Proteintech, #16,488–1-AP, Wuhan, China, 1:250), anti-KLF4 (Proteintech, #11,880–1-AP, Wuhan, China, 1:200), anti-CDX2 (Immunoway, #YM3057, Jiangsu, China, 1:200), and anti-MUC2 (Proteintech, #27,675–1-AP, Wuhan, China, 1:400), were applied, and plates were sealed with parafilm and incubated overnight at 4 °C. .. The next day, cells were incubated in the dark for 1 h with Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (H + L) (Thermo Fisher Scientific, #A-11008, USA, 4 μg/mL) or Alexa Fluor 594-conjugated Goat anti-Mouse IgG (H + L) (Thermo Fisher Scientific, #A-11005, USA, 5 μg/mL) cross-adsorbed secondary antibody.



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